The specific immunogen used to produce this antibody is proprietary information.

Endogenous

CHEK2

Serine/threonine-protein kinase Chk2

CHEK2 ;
CDS1 ;
CHK2 ;
RAD53 ;
Serine/threonine-protein kinase Chk2 ;
CHK2 checkpoint homolog ;
Cds1 homolog ;
Hucds1 ;
hCds1 ;
Checkpoint kinase 2

In response to DNA damage and replication blocks , cell cycle progression is halted through the control of critical cell cycle regulators. The protein encoded by this gene is a cell cycle checkpoint regulator and putative tumor suppressor. It contains a forkhead-associated protein interaction domain essential for activation in response to DNA damage and is rapidly phosphorylated in response to replication blocks and DNA damage. When activated , the encoded protein is known to inhibit CDC25C phosphatase , preventing entry into mitosis , and has been shown to stabilize the tumor suppressor protein p53 , leading to cell cycle arrest in G1. In addition , this protein interacts with and phosphorylates BRCA1 , allowing BRCA1 to restore survival after DNA damage. Mutations in this gene have been linked with Li-Fraumeni syndrome , a highly penetrant familial cancer phenotype usually associated with inherited mutati

Catalytic activity:ATP + a protein = ADP + a phosphoprotein. ,cofactor:Magnesium. ,Disease:Defects in CHEK2 are associated with Li-Fraumeni syndrome 2 (LFS2) [MIM:609265]; a highly penetrant familial cancer phenotype usually associated with inherited mutations in p53/TP53. ,Disease:Defects in CHEK2 are found in some patients with osteosarcoma (OSRC) [MIM:259500]. ,Disease:Defects in CHEK2 are found in some patients with prostate cancer (CaP) [MIM:176807]. ,enzyme regulation:Rapidly phosphorylated on Thr-68 by MLTK in response to DNA damage and to replication block. Kinase activity is also up-regulated by autophosphorylation. ,Function:Regulates cell cycle checkpoints and apoptosis in response to DNA damage , particularly to DNA double-strand breaks. Inhibits CDC25C phosphatase by phosphorylation on 'Ser-216' , preventing the entry into mitosis. May also play a role in meiosis. Regulates the TP53 tumor suppressor through phosphorylation at 'Thr-18' and 'Ser-20'. ,similarity:Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CHK2 subfamily. ,similarity:Contains 1 FHA domain. ,similarity:Contains 1 protein kinase domain. ,subcellular location:Isoform 10 is present throughout the cell. ,tissue specificity:High expression is found in testis , spleen , colon and peripheral blood leukocytes. Low expression is found in other tissues. ,

Nucleus

>>Cell cycle ;
>>p53 signaling pathway ;
>>Cellular senescence ;
>>Human T-cell leukemia virus 1 infection