The antiserum was produced against synthesized peptide derived from human EIF2AK3. AA range:947-996

PERK Polyclonal Antibody detects endogenous levels of PERK protein.

EIF2AK3

Eukaryotic translation initiation factor 2-alpha kinase 3

EIF2AK3 ;
PEK ;
PERK ;
Eukaryotic translation initiation factor 2-alpha kinase 3 ;
PRKR-like endoplasmic reticulum kinase ;
Pancreatic eIF2-alpha kinase ;
HsPEK

The protein encoded by this gene phosphorylates the alpha subunit of eukaryotic translation-initiation factor 2 , leading to its inactivation , and thus to a rapid reduction of translational initiation and repression of global protein synthesis. This protein is thought to modulate mitochondrial function. It is a type I membrane protein located in the endoplasmic reticulum (ER) , where it is induced by ER stress caused by malfolded proteins. Mutations in this gene are associated with Wolcott-Rallison syndrome. [provided by RefSeq , Sep 2015] ,

Catalytic activity:ATP + a protein = ADP + a phosphoprotein. ,Disease:Defects in EIF2AK3 are the cause of Wolcott-Rallison syndrome (WRS) [MIM:226980]; also known as multiple epiphyseal dysplasia with early-onset diabetes mellitus. WRS is a rare autosomal recessive disorder , characterized by permanent neonatal or early infancy insulin-dependent diabetes and , at a later age , epiphyseal dysplasia , osteoporosis , growth retardation and other multisystem manifestations , such as hepatic and renal dysfunctions , mental retardation and cardiovascular abnormalities. ,Domain:The lumenal domain senses perturbations in protein folding in the ER , probably through reversible interaction with HSPA5/BIP. ,enzyme regulation:Perturbation in protein folding in the endoplasmic reticulum (ER) promotes reversible dissociation from HSPA5/BIP and oligomerization , resulting in transautophosphorylation and kinase activity induction. ,Function:Phosphorylates the alpha subunit of eukaryotic translation-initiation factor 2 (EIF2) , leading to its inactivation and thus to a rapid reduction of translational initiation and repression of global protein synthesis. Serves as a critical effector of unfolded protein response (UPR) -induced G1 growth arrest due to the loss of cyclin D1. ,induction:By ER stress. ,PTM:Autophosphorylated. ,PTM:N-glycosylated. ,similarity:Belongs to the protein kinase superfamily. ,similarity:Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. GCN2 subfamily. ,similarity:Contains 1 protein kinase domain. ,subunit:Forms dimers with HSPA5/BIP in resting cells. Oligomerizes in ER-stressed cells. Interacts with DNAJC3. ,tissue specificity:Ubiquitous. A high level expression is seen in secretory tissues. ,

Endoplasmic reticulum membrane; Single-pass type I membrane protein.

>>Mitophagy - animal ;
>>Autophagy - animal ;
>>Protein processing in endoplasmic reticulum ;
>>Apoptosis ;
>>Non-alcoholic fatty liver disease ;
>>Alzheimer disease ;
>>Parkinson disease ;
>>Amyotrophic lateral sclerosis ;
>>Prion disease ;
>>Pathways of neurodegeneration - multiple diseases ;
>>Hepatitis C ;
>>Measles ;
>>Herpes simplex virus 1 infection ;
>>Lipid and atherosclerosis